FastQCFastQC Report
Sat 18 Mar 2023
SRR4895628_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895628_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618946
Sequences flagged as poor quality0
Sequence length151
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACAGA624250.0142.979021
CACAGAA624500.0142.921782
ACAGAAT217900.0142.758933
CAGAATG75750.0142.501544
CAGAAGT71450.0142.29754
ACAGAAG207350.0141.328583
CAGAATA66700.0139.78264
CAGAATT75950.0139.3734
CAGAAAT67450.0138.33524
CAGAAGG77650.0137.714614
CAGAAAG74400.0136.849154
CAGAAGA60300.0134.170154
ACAGAAA201500.0132.155323
AGAAGTT25100.0126.403575
AGAATTG29350.0126.034565
AGAATGG27350.0124.968745
AGAAGGT24600.0124.8690645
AGAATGT28550.0124.262295
AGAAGTG26200.0122.7478565
CAGAACT5300.0121.086174
Produced by FastQC (version 0.11.5)