FastQCFastQC Report
Sat 18 Mar 2023
SRR4895628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618946
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG162642.6276928843550165No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30640.49503510807081713No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA23110.3733766758327867No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT10250.16560410762812913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC9120.14734726454327193No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG7090.1145495729837498No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC6910.11164140328881679No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG6800.10986418847524662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT163650.081.7437741
AGTGGTA165500.080.122585
GGTATCA166900.079.617818
GTATCAA167550.079.568559
AGCAGTG167550.079.531712
GTGGTAT167250.079.191136
CAGTGGT168650.078.712054
TGGTATC169800.077.959177
GCAGTGG173250.076.58033
CGGTCTG2550.036.960888145
GGTCTGA4600.033.09792145
GCCCCAA6200.029.233953145
CATGCGA1450.00122589525.00007145
CGACCTA3350.023.80411545-49
TACGCAC3450.023.1141450-54
CATGGGG38950.023.0057425-29
CGCGAAA2450.022.48804390-94
TCCGGCG3550.021.64899635-39
TCTCGCA4000.021.02675670-74
CTCGTTG2250.020.6232835-39
Produced by FastQC (version 0.11.5)