FastQCFastQC Report
Sat 18 Mar 2023
SRR4895627_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895627_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539715
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACA541050.0143.867752
AAGGTAC541300.0143.80131
GGTACAT196900.0143.678253
GGTACAG177450.0143.455493
GTACAGT62350.0143.302354
GTACATG65250.0142.90144
GGTACAA158850.0142.82033
GTACAGG60000.0142.665154
GTACATA62400.0142.378564
GTACATT69050.0142.138894
GTACAAT59150.0142.033134
GTACAAG54450.0141.976144
GTACAGA54600.0141.586094
TACAGTG20750.0141.09985
TACAATG21200.0139.805565
TACAGTA19850.0139.141515
TACATAG22450.0138.124455
TACATGT22300.0138.08345
TACATTG26200.0137.621785
ACAGATG6300.0137.359656
Produced by FastQC (version 0.11.5)