FastQCFastQC Report
Sat 18 Mar 2023
SRR4895627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539715
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG104391.9341689595434628No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24340.4509787573070973No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13020.24123843139434703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT120300.086.66031
GTGGTAT120250.086.093446
AGTGGTA120400.085.9861765
GGTATCA122700.085.142498
TGGTATC122400.084.699637
AGCAGTG123400.084.248252
CAGTGGT123000.084.2275164
GTATCAA124650.084.101349
GCAGTGG127300.081.32553
CGGTCTG1950.052.04995145
ACGTACC1050.00770632127.6183411
GCTATCC1958.7464705E-626.024975145
AGGTACA2601.1429984E-725.0955122
CGATAGG1400.023.8208235-39
TACGCAC1100.023.72666550-54
CGTTCAA1902.1917315E-422.894152145
CATGGGG32050.022.71080825-29
AGGTCCG604.557096E-721.751863120-124
CGATAGA604.558733E-721.751057105-109
TTACGGG403.1054646E-421.74944385-89
Produced by FastQC (version 0.11.5)