FastQCFastQC Report
Sat 18 Mar 2023
SRR4895626_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895626_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708780
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG76821.0838341939670986No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35150.495922571178645No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA10280.14503795253816418No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC8050.11357543948757019No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG7440.10496910183695929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT137500.097.020351
AGTGGTA137600.096.278465
GGTATCA138400.095.977138
GTGGTAT138000.095.8875356
GTATCAA140250.094.749449
TGGTATC140050.094.5357367
CAGTGGT140250.094.3041844
AGCAGTG141050.094.28342
GCAGTGG145850.090.683323
CGGTCTG2450.056.22575145
CGACCTA1900.024.41987645-49
CGGAGCC3352.1063897E-923.806503145
CGCGAAA1750.023.20019390-94
CATGGGG37950.022.88655925-29
TACGCAC2300.022.06415450-54
CCCGCGG1700.003091514321.324007145
CTCGTTG2050.021.21938935-39
TCCGGCG2000.021.02487835-39
CCGCGAA1950.020.82068490-94
TACATGG134400.020.66051320-24
Produced by FastQC (version 0.11.5)