FastQCFastQC Report
Sat 18 Mar 2023
SRR4895625_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895625_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394792
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCTTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT4540.11499726438225699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA395150.0144.091862
CGCTTAT134450.0143.916023
CGCTTAG131350.0143.743713
CGCTTAA123350.0143.536273
AACGCTT396900.0143.456531
GCTTAAG42450.0142.708854
GCTTAGT44050.0142.273224
GCTTATG49450.0142.050234
GCTTAGG47800.0141.672974
GCTTATA40150.0141.184274
GCTTAGA39400.0141.126144
CTTAGTG13350.0140.996575
GCTTAAT43650.0140.768234
CTTAGAG13100.0140.384195
GCTTATT46150.0138.924674
GCTTAAA38600.0138.81964
CTTATAG14700.0138.350625
CTTAAGG15000.0137.506795
CGCTTAC7300.0137.322363
CTTAGTA15700.0136.428825
Produced by FastQC (version 0.11.5)