FastQCFastQC Report
Sat 18 Mar 2023
SRR4895624_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895624_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285273
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGACGC100.007197816144.257778
CCGAGAT97950.0144.059953
CGAGATA31750.0143.980094
ACCGAGA285750.0143.954852
AACCGAG285900.0143.879331
CGAGAGT35150.0143.796924
CGAGATG36050.0143.607154
CCGAGAA84350.0143.352373
CCGAGAG100500.0143.346253
CGAGATT30000.0143.005464
CGAGAAT30450.0142.786424
CGAGAGA29250.0141.49564
CGAGAAG29450.0140.779514
CGAGAGG35850.0139.983554
CGAGACT1000.0136.996834
CGAGAAA25950.0135.87154
CGAGATC550.0131.097464
CCGAGAC4100.0128.379583
AGATGTG5000.0128.34446
GAGATGT14850.0127.698625
Produced by FastQC (version 0.11.5)