FastQCFastQC Report
Sat 18 Mar 2023
SRR4895624_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895624_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285273
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG56591.9837138460352015No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10680.374378227171867No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG8070.28288691884615785No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA7460.2615038927623715No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC4800.16825987738061435No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC4800.16825987738061435No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3190.11182271017586662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT83150.099.724531
GGTATCA84900.097.754348
AGCAGTG84800.097.613192
GTGGTAT84750.097.4997256
CAGTGGT85100.096.928384
TGGTATC85750.096.616297
AGTGGTA85450.096.616195
GTATCAA86450.096.2700659
GCAGTGG87400.094.04593
AACCGAG902.046363E-856.375941
CCGCAAG2250.051.543713145
ACCGAGA1109.9436875E-846.1257672
CACGGGA907.4790965E-540.268528145
TACGCAC459.1024776E-725.78089550-54
CATGGGG22750.024.60172325-29
TGCGGCT2101.5527716E-524.161118145
ATCTCAC1500.001493658224.1611164
CGACCTA502.2828208E-623.19711145-49
ATAGGCG1200.022.965137100-104
ATGGGGG10050.022.50702725-29
Produced by FastQC (version 0.11.5)