FastQCFastQC Report
Sat 18 Mar 2023
SRR4895623_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895623_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068466
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT703866.587575084279705No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT152681.4289645154829447No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT82940.7762530581225795No Hit
AACAACCATGTACTCTGCGTGGATACCACTGCTTCCCATGTACTCTGCGT28030.26233871737612613No Hit
AACAACCATGTACTCTGCGTTGAGACCACTGCTTCCCATGTACTCTGCGT24180.226305750487147No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGCCGC22250.20824247098176263No Hit
AACAACCAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG20600.19279977088648587No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGC18540.17351979379783727No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGGC15060.1409497354150717No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTC14760.138141971761385No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGCGGC13480.1261621801723218No Hit
AACAACCATGTACTCCGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12830.12007869225600067No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCGTCCCATGTACTCTGCGT12200.11418238858325863No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGT11980.11212336190388839No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCAGGAACCCGCCGT11630.10884763764125391No Hit
AACAACCATGTACTCTGCGTTGATACGACTGCTTCCCATGTACTCTGCGT11550.10809890066693746No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGC10690.10004997819303561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCAT492550.0143.033813
AACCATG301400.0142.951644
CAACCAG310950.0142.549963
ACCATGT229350.0142.490425
ACAACCA1083250.0142.249252
AACAACC1087600.0141.68031
AACCAGT109450.0141.25494
ACCATAG33550.0139.706075
ACCAGTT33950.0138.272485
ACCAGAT32800.0137.624255
ACCAGTA35050.0137.22475
ACCATGA32200.0136.381645
AACCATA94350.0135.965444
AACCAGA97700.0135.731814
ACCAATG35500.0135.688375
CCATATG10850.0135.579776
ACCAGGT36300.0135.479085
AACCATT103550.0135.445314
ACCAGTG38000.0135.111055
AACCAGG112200.0135.029194
Produced by FastQC (version 0.11.5)