FastQCFastQC Report
Sat 18 Mar 2023
SRR4895623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068466
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG14528613.597625006317468No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA226982.1243539803793476No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG51500.48199942721621464No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44140.4131156255791013No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA35510.3323456244747142No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTTGTTAGATCGGAAGAGCACA29600.27703268049708646No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA24640.2306109880894666No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC19190.17960328171415843No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG18720.17520445199004928No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA15070.14104332753686127No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT14300.1338367341590654No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT13620.1274724698773756No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG13020.12185694257000222No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC12550.11745811284589308No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG12480.1168029679933662No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA12010.11240413826925705No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG11980.11212336190388839No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC11940.11174899341673016No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11850.11090666432062414No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11770.1101579273463077No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10850.10154745214166852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT991750.059.079531
AGCAGTG1031650.056.7524152
AGTGGTA1073750.054.4597175
CAGTGGT1079450.054.178864
GTGGTAT1084950.053.864116
TGGTATC1088050.053.7106487
GCAGTGG1090800.053.5619433
GGTATCA1096800.053.506928
GTATCAA1101250.053.3556379
CATGCGT2700.048.332836145
CATGCGA3000.036.249626145
CGGGGTT2451.8189894E-1235.50984145
CGTGGTT5850.034.700497145
CGAACTC3200.033.984024145
CCGGGTT5750.032.782272145
CGTGCTC10900.032.591404145
CATGCGG2051.1388693E-831.82894145
CCGGCTT12800.031.718422145
CCGTCTT34850.031.620907145
AAATATA24150.030.020393145
Produced by FastQC (version 0.11.5)