FastQCFastQC Report
Sat 18 Mar 2023
SRR4895622_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895622_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442886
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTACAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG6480.1463130466982474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG444000.0143.827152
AGTACAA445050.0143.487811
TACAAGT146850.0142.973183
TACAAGA134800.0141.90073
ACAAGAT46150.0140.91984
TACAAGG150950.0140.809363
ACAAGTG50350.0140.763874
CAAGATG16500.0139.830925
ACAAGTA47700.0139.817184
ACAAGTT50050.0139.30274
CAAGTTG19300.0138.970555
TACAAGC17950.0138.175543
ACAAGGT55300.0138.072754
CAAGGTG18450.0137.166465
CAAGTAG18300.0137.10885
CAAGAGT15200.0137.085465
CAAGTGA16300.0136.238635
CAAGGTA17800.0136.099475
CAAGATA14950.0136.001945
CAAGTGT14950.0135.519655
Produced by FastQC (version 0.11.5)