FastQCFastQC Report
Sat 18 Mar 2023
SRR4895622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442886
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG137453.1035074488694607No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA19050.43013326228419957No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17790.4016835032039848No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC7390.16686009492284695No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG7300.1648279692742602No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT6730.15195784016654398No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC4520.1020578659068022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT146050.077.536221
GTATCAA148750.076.0313649
AGTGGTA149150.075.738795
GGTATCA149450.075.732318
GTGGTAT149650.075.437296
CAGTGGT150300.075.207534
AGCAGTG150600.075.154012
TGGTATC150950.074.835657
GCAGTGG153950.073.2360463
CGGTCTG1450.055.00458145
CCGCAAG4800.045.316277145
CGCCGAA657.329128E-444.619102145
CGCTGTT2252.0008883E-1135.447395145
CGGAGCC2003.245059E-729.002417145
TACGCAC1400.026.92777650-54
AACGGCA1100.00966195326.3658334
CGCGAAA1100.025.04471490-94
CATGGGG30750.023.34077325-29
GGTCTGA2502.2728036E-623.201935145
CGACCTA1450.022.99931745-49
Produced by FastQC (version 0.11.5)