FastQCFastQC Report
Sat 18 Mar 2023
SRR4895621_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895621_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785030
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGAT785600.0144.091662
AACGTGA785950.0144.027481
CGTGATA189500.0143.935443
CGTGATG347950.0143.766633
CGTGATT220950.0143.516513
CGTGATC29550.0141.761833
GTGATGA87750.0141.24384
GTGATGT165800.0140.809834
GTGATAG49100.0139.062214
GTGATGG98800.0138.87454
GTGATTA66900.0138.274224
GTGATAT91400.0138.206514
GTGATAA55150.0137.142044
TGATGTG72400.0135.438145
TGATGAG35950.0135.37725
GTGATTG54800.0134.860244
TGATTAG26600.0133.63065
TGATATG36350.0131.110575
TGATGTA39800.0130.433765
GATGTAG15100.0129.877096
Produced by FastQC (version 0.11.5)