FastQCFastQC Report
Sat 18 Mar 2023
SRR4895621_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895621_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785030
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG200352.5521317656650067No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45950.5853279492503471No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA27810.3542539775549979No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11080.14114110288778772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT193600.078.239841
AGTGGTA194100.077.632325
GGTATCA199400.076.1458748
GTGGTAT199800.075.4079746
GTATCAA201450.075.36149
CAGTGGT200600.075.225264
AGCAGTG201500.075.105192
TGGTATC201650.074.7568747
GCAGTGG204900.073.646593
CGGAGCC2755.456968E-1231.63446145
CGGTCTG2301.0022632E-931.519842145
CGCTGTT4450.029.324076145
GCTATCC3503.6379788E-1226.92695145
CGTGATT1358.071974E-426.8570773
CGATAGG2450.024.265735-39
GGTCGCG3100.022.91914450-54
TACGCAC2100.022.78550550-54
AGGTATC2903.2800563E-722.5029457
GAGGTAT2953.8696817E-722.120136
AACGTGA4607.2759576E-1222.0681061
Produced by FastQC (version 0.11.5)