FastQCFastQC Report
Sat 18 Mar 2023
SRR4895620_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895620_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517632
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5617010.851338402571711No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA86461.6702985905044512No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG21860.42230773986152326No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA13650.263700853115727No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11870.22931348911968347No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC10680.20632418397626115No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA8940.1727095697329377No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG7230.13967451780415432No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC6720.12982195845697328No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT6350.12267402324431255No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA6090.11765114985163205No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT5820.11243508902077151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAA554.0596096E-665.90698145
AAGCAGT376700.056.0728841
AGCAGTG399600.052.799962
AGTGGTA400100.052.6252445
CAGTGGT407650.051.75734
GCAGTGG413050.050.9402243
GTGGTAT414950.050.684616
GTATCAA417650.050.530539
TGGTATC417200.050.4807667
GGTATCA418000.050.4708758
TGCGGCT2900.044.99856145
CATGCGA907.4788426E-540.27649145
AATCGCC951.0283973E-438.156673145
CGGTCTG800.002036028836.24884145
CGTGGGA3050.033.277626145
CGGAGCC2558.731149E-1131.27351145
CACGGGC2401.5788828E-930.207365145
AAATATA11350.030.021067145
GCGTAGG200.006077165228.999073110-114
CGTGGGC3550.028.590633145
Produced by FastQC (version 0.11.5)