FastQCFastQC Report
Sat 18 Mar 2023
SRR4895619_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895619_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682551
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGCTA683850.0143.957812
AAGCTAG256850.0143.726613
AGCTAGT85850.0143.643984
ACAAGCT686000.0143.506641
AAGCTAT224950.0143.302293
AGCTATG95250.0143.096314
AGCTAAT63500.0142.982744
AGCTAGG95450.0142.720934
AGCTAAG74900.0142.595184
AGCTAGA74350.0142.486084
AAGCTAA195550.0142.424963
CTAGATA7600.0142.334216
GCTAGAT24950.0142.20875
AGCTATA66500.0142.171554
GCTATAG26650.0142.067155
GCTAGGT32550.0141.351785
GCTAAGT23450.0141.15675
GCTAATG26750.0140.996895
GCTAGAG26500.0140.966375
GCTATGG38100.0140.824955
Produced by FastQC (version 0.11.5)