FastQCFastQC Report
Sat 18 Mar 2023
SRR4895618_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895618_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3985736
Sequences flagged as poor quality0
Sequence length150
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAT3987400.0143.937911
GCTGATC3988500.0143.898222
CTGATCG1449800.0143.840383
CTGATCA1181300.0143.712843
TGATCGA431350.0143.515244
CTGATCT1357100.0143.399783
TGATCGG493000.0143.34214
TGATCAG439650.0143.180474
TGATCGT525550.0143.16364
GATCGAG146250.0142.916235
GATCGAT156750.0142.896915
TGATCTA392650.0142.587364
GATCGAA130000.0142.448555
GATCGGT183850.0142.354495
TGATCTG534400.0142.342124
TGATCAT394900.0142.194294
GATCTAG161700.0142.084665
GATCGTG184400.0142.047035
GATCGTA158600.0142.001835
GATCATG158400.0141.862955
Produced by FastQC (version 0.11.5)