FastQCFastQC Report
Sat 18 Mar 2023
SRR4895618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3985736
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA128270.3218226194609979No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG92770.23275500434549604No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC88280.22148983274356354No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA59990.15051172481067487No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCTGTGGCTCAC56450.14163005276817128No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGTGGTGGCTCA55070.1381677060397377No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG53740.1348308066565372No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC49210.12346527717841825No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG48130.12075561452138325No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGTGGTGGCTCAC45930.11523593133112679No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCGCTGTGGCTCACG45610.11443306832163494No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCCAGGCATGGTGGCTC43220.1084366852194927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT916050.0135.640876
GGTATCA920500.0135.08688
AAGCAGT922400.0134.916531
TGGTATC924900.0134.389667
AGTGGTA925400.0134.324845
GTATCAA930500.0133.720149
AGCAGTG938850.0132.426832
CAGTGGT944250.0131.677124
GCAGTGG968800.0128.303193
CAACGCA911400.027.95539510-14
TCAACGC913800.027.85045810-14
ATCAACG917900.027.6993910-14
GTACATG918500.027.59298320-24
TACATGG919100.027.578120-24
CGCAGAG923150.027.5329215-19
AACGCAG924900.027.46328710-14
AGAGTAC925450.027.36802915-19
AGTACAT925750.027.36133220-24
CAGAGTA930650.027.30948815-19
GAGTACA916350.027.1753120-24
Produced by FastQC (version 0.11.5)