FastQCFastQC Report
Sat 18 Mar 2023
SRR4895617_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895617_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933548
Sequences flagged as poor quality0
Sequence length150
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCAAG2936650.0143.844791
ATCAAGT2937250.0143.81542
TCAAGTA825450.0143.318913
TCAAGTG1148400.0142.808033
CAAGTAG322950.0142.416354
CAAGTAT262350.0142.160494
CAAGTGT407950.0141.969594
TCAAGTT983050.0141.67753
CAAGTGG426900.0141.604324
CAAGTTG414200.0140.922494
CAAGTGA320300.0140.515034
CAAGTAA247550.0140.363634
CAAGTTA284550.0140.254414
AAGTGAG119200.0133.730835
CAAGTTT306000.0133.246374
AAGTGTA130250.0132.888375
AAGTTAG124800.0132.518545
AAGTAGT123600.0131.591555
AAGTATG121800.0131.46735
AAGTAGG128450.0131.387395
Produced by FastQC (version 0.11.5)