FastQCFastQC Report
Sat 18 Mar 2023
SRR4895617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933548
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG143300.4884869789074527No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA108310.3692116167862261No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC89240.3042050104515079No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA74060.2524587973334679No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC55960.1907587671993095No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG50340.17160107828472554No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT42400.14453487722034888No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA37720.1285814992630085No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC30880.10526502378689559No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC30360.10349242623608No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG29570.10079944149541784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT755150.0128.535746
GGTATCA756800.0128.322088
AAGCAGT758250.0128.257021
TGGTATC758200.0128.047167
AGTGGTA761700.0127.5154955
GTATCAA764550.0127.0966649
AGCAGTG773000.0125.595552
CAGTGGT774250.0125.457864
GCAGTGG793500.0122.3054353
CGATAGG19800.027.41677535-39
CAACGCA753900.026.44689610-14
TCAACGC754950.026.37387710-14
ATCAACG756400.026.30428110-14
GTACATG751900.026.29025320-24
CATGGGG379600.026.26452625-29
TACATGG753850.026.22988920-24
AGAGTAC757650.026.08449415-19
CGCAGAG761050.026.05499515-19
CAGAGTA761650.026.04581315-19
AACGCAG762600.026.0451110-14
Produced by FastQC (version 0.11.5)