FastQCFastQC Report
Sat 18 Mar 2023
SRR4895616_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895616_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5306568
Sequences flagged as poor quality0
Sequence length150
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTG5313900.0143.799842
CAGATCT5314400.0143.78631
GATCTGG2036300.0143.521673
GATCTGA1503950.0143.43413
GATCTGT1773550.0143.426593
ATCTGAG588950.0142.814514
ATCTGGA555100.0142.807054
ATCTGAT485450.0142.650674
ATCTGTG745750.0142.108034
ATCTGGT700750.0142.007054
ATCTGTA503900.0141.971394
ATCTGGG794750.0141.545234
ATCTGAA440850.0140.227654
TCTGAGT208450.0138.853795
ATCTGTT550800.0138.784654
TCTGAGG237300.0138.629935
TCTGATG211400.0138.550965
TCTGGTA206450.0138.176185
TCTGGAG219850.0138.170915
TCTGAGA160700.0137.638175
Produced by FastQC (version 0.11.5)