FastQCFastQC Report
Sat 18 Mar 2023
SRR4895615_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895615_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803681
Sequences flagged as poor quality0
Sequence length150
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGGC1806650.0143.76042
ACATTGG1807600.0143.684861
ATTGGCG687950.0143.539033
ATTGGCT607800.0142.980793
ATTGGCA514700.0142.740553
TTGGCGA199050.0141.61224
TGGCGTA70800.0141.558855
TGGCGAT67600.0141.12385
TTGGCTA174750.0140.827014
TGGCTAG70450.0140.729145
TTGGCGT243650.0140.335274
TTGGCGG258300.0139.846224
TTGGCTG249000.0139.575444
TGGCGGA70250.0139.284945
TTGGCAT170750.0139.234684
TGGCGAA60300.0139.104025
TGGCGAG73550.0138.713355
GGCGTAT23200.0138.413356
GGCGATA21750.0138.040926
GGCGTAG28150.0137.8616
Produced by FastQC (version 0.11.5)