FastQCFastQC Report
Sat 18 Mar 2023
SRR4895615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803681
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55840.3095891124871859No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG46620.25847142593396505No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC46340.2569190449974247No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC35210.19521190276994657No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG31870.17669421588407264No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA27510.15252142701508747No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT25300.14026870605167988No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG25190.13965884211232474No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC23830.1321187061348431No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA23020.12762788985413717No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC21220.11764829811923506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT421550.0132.857546
AAGCAGT423100.0132.501171
GGTATCA423600.0132.367558
AGTGGTA426200.0131.441795
TGGTATC426950.0131.261477
GTATCAA427900.0131.037379
CAGTGGT434550.0128.949234
AGCAGTG435550.0128.686232
GCAGTGG441950.0126.6923753
CAACGCA419900.027.30346710-14
GTACATG418000.027.17685720-24
ATCAACG422400.027.0805110-14
TCAACGC423100.027.06293110-14
TACATGG420500.027.04610320-24
AGAGTAC422600.026.92488515-19
AGTACAT421500.026.92385720-24
CGCAGAG424000.026.89711215-19
CAGAGTA424800.026.87696515-19
AACGCAG425800.026.83383610-14
GCAGAGT426800.026.74089615-19
Produced by FastQC (version 0.11.5)