FastQCFastQC Report
Sat 18 Mar 2023
SRR4895614_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895614_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4859019
Sequences flagged as poor quality0
Sequence length150
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTGT4870350.0143.66372
ACCACTG4875500.0143.511951
CACTGTA1528600.0143.44823
CACTGTG1795400.0143.333573
CACTGTT1555850.0142.694273
ACTGTAG567950.0142.680864
ACTGTAA501300.0141.95984
ACTGTGT614250.0141.865954
ACTGTTA479300.0141.761034
ACTGTGA565150.0141.731584
ACTGTAT470450.0141.703634
ACTGTTG639850.0141.591234
ACTGTGG634100.0141.149284
CTGTATG200900.0141.132195
CTGTTAG200600.0140.26655
CTGTGAG197500.0139.697535
CTGTAGT193400.0138.973455
CTGTAGA182850.0138.919745
CTGTGGA198400.0138.846085
CTGTGTA200300.0138.823075
Produced by FastQC (version 0.11.5)