FastQCFastQC Report
Sat 18 Mar 2023
SRR4895614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4859019
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA174630.3593935319042794No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG109550.22545703155307686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC90110.18544895584890694No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGTGGTGGCTCACG88030.1811682563908476No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG84980.174891269204751No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCAGTGGCTCAC82610.1700137414568661No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG80510.16569188142709465No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGTGGTGGCTCAC79430.16346921055464075No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCGCAGTGGCTCACG75830.15606030764646117No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCACGGTGGCTCA72680.149577517601804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCT71390.14692266072637297No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC59920.12331707284947847No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA54160.11146282819639108No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC53880.11088658019242155No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCAGTGGCTCACG52950.10897261360780848No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC52330.10769663588473312No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT51260.10549454529813528No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC50660.10425972814677202No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCTT49670.10222227984702263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1114450.0134.46366
AAGCAGT1116500.0134.39641
GGTATCA1116900.0134.233118
TGGTATC1122100.0133.585397
GTATCAA1129400.0132.830299
AGCAGTG1146000.0130.832182
AGTGGTA1154050.0129.899525
CAGTGGT1173650.0127.774454
GCAGTGG1205600.0124.3404853
CAACGCA1110600.027.6860710-14
TCAACGC1112700.027.61311110-14
ATCAACG1116350.027.48026310-14
GTACATG1112450.027.47232620-24
TACATGG1118150.027.35159720-24
AGAGTAC1114050.027.33688415-19
CGCAGAG1119900.027.32394815-19
CAGAGTA1121850.027.27901815-19
AGTACAT1119250.027.2668220-24
AACGCAG1126000.027.1846510-14
CGATAGG22850.027.09732635-39
Produced by FastQC (version 0.11.5)