FastQCFastQC Report
Sat 18 Mar 2023
SRR4895613_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895613_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3129243
Sequences flagged as poor quality0
Sequence length150
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTCA3134300.0143.764982
AGTGGTC3134800.0143.742051
TGGTCAG1208600.0143.379753
TGGTCAT966300.0143.291433
TGGTCAA967550.0142.99473
GGTCATA287300.0142.721194
GGTCATG408350.0142.712174
GTCATAG128150.0142.31385
GGTCAGA349600.0142.228154
GGTCAGT410250.0142.209174
GGTCAAT311900.0141.852464
GTCATGA119100.0141.27895
GTCAGAT115650.0140.948735
GGTCAGG459650.0140.913484
GTCATGG165550.0140.693975
GGTCATT277550.0140.393484
GTCAATG134850.0140.155035
GTCAAGA108000.0140.132665
GTCAGGA140600.0139.95385
GTCAGGT154800.0139.95285
Produced by FastQC (version 0.11.5)