FastQCFastQC Report
Sat 18 Mar 2023
SRR4895613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3129243
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG133700.4272598836204155No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA109330.34938162360673175No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA77640.2481111246394096No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC66900.21378972486316977No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC60200.1923787957662604No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC58140.18579573398422558No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG50590.16166849298696204No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC50320.16080566450096717No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC47880.15300825151642106No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA47800.15275259863168184No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG45860.14655301617675584No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCAGTGGCTCA40690.13003144850048398No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA40600.12974383900515235No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT37150.11871880835077366No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT36590.11692923815759915No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC36500.11664162866226752No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCACGGTGGCTCA33540.10718247192691652No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCACGGTGGCTCAT32330.10331572204523587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT787400.0135.786931
GTGGTAT787500.0135.537256
GGTATCA789950.0135.226268
TGGTATC793550.0134.567437
GTATCAA796300.0134.238339
AGCAGTG802750.0133.096952
AGTGGTA803050.0132.975515
CAGTGGT803850.0132.914834
GCAGTGG820750.0130.116563
CAACGCA783800.027.9923110-14
TCAACGC786100.027.88293310-14
GTACATG782650.027.84806320-24
ATCAACG790000.027.70700610-14
TACATGG785650.027.70690220-24
TAAGCAG16450.027.7065831
AGTACAT791350.027.54008320-24
CGCAGAG794850.027.51275615-19
CAGAGTA794600.027.51235415-19
AGAGTAC792650.027.48193715-19
GCAGAGT798000.027.38791315-19
Produced by FastQC (version 0.11.5)