FastQCFastQC Report
Sat 18 Mar 2023
SRR4895612_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895612_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3593363
Sequences flagged as poor quality0
Sequence length150
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA3595650.0143.905911
TGCCTAA3598150.0143.805922
GCCTAAG1391600.0143.703123
GCCTAAT1153900.0143.480133
CCTAAGT465950.0143.044
GCCTAAA1057050.0143.037613
CCTAAGG534600.0142.987954
CCTAATG508900.0142.866184
CCTAAGA395500.0142.614474
CTAATGG197800.0142.360035
CCTAAAG437750.0142.007874
CTAAGTG179800.0141.595385
CTAAGGT191500.0141.403815
CCTAATA328100.0141.342774
CTAATAG143600.0141.340675
CTAATGA141050.0140.629045
CTAAGGA157500.0140.432365
CTAAGTA141650.0140.389185
CTAAGAT135550.0139.217585
CTAAGGG195150.0138.869745
Produced by FastQC (version 0.11.5)