FastQCFastQC Report
Sat 18 Mar 2023
SRR4895612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3593363
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG183500.5106636874704837No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121050.33687105922780414No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC113370.31549832288026564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC79520.22129687426513828No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG72280.20114861760417746No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC59900.166696211877286No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA48620.13530500536683882No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC43670.12152960889283938No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC38110.10605663830790266No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC37210.10355202076717548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA799500.0132.834238
GTGGTAT798850.0132.816156
AAGCAGT802900.0132.120351
TGGTATC806500.0131.690237
AGTGGTA806600.0131.60255
GTATCAA810950.0131.029749
AGCAGTG819350.0129.43162
CAGTGGT821100.0129.33114
GCAGTGG839650.0126.328083
CAACGCA793650.027.45573210-14
TAAGCAG21400.027.3810391
TCAACGC798900.027.2482710-14
GTACATG796200.027.21597120-24
ATCAACG800450.027.1415410-14
TACATGG798900.027.12399320-24
AACGCAG802900.027.0013310-14
CGCAGAG805500.026.94888915-19
AGTACAT807200.026.83260220-24
AGAGTAC808100.026.7855615-19
CAGAGTA812350.026.77837215-19
Produced by FastQC (version 0.11.5)