FastQCFastQC Report
Sat 18 Mar 2023
SRR4895611_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895611_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2657477
Sequences flagged as poor quality0
Sequence length150
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCA2658800.0143.92632
AGATCGC2658950.0143.918181
ATCGCAT962550.0143.842353
ATCGCAG884800.0143.795993
ATCGCAA808800.0143.785773
TCGCAGT320800.0143.505654
CGCATAG100900.0143.499925
TCGCATG314800.0143.29044
TCGCATA297750.0143.24984
TCGCAGA257650.0143.216964
TCGCAAG265550.0143.1864
TCGCATT352700.0143.019554
CGCAATG101600.0142.79475
CGCATGA87800.0142.687365
CGCATTG122500.0142.58885
CGCATGT116700.0142.58045
CGCATGG111550.0142.579445
TCGCAGG308600.0142.506234
TCGCAAT295750.0142.490054
CGCAGAT93750.0142.233025
Produced by FastQC (version 0.11.5)