FastQCFastQC Report
Sat 18 Mar 2023
SRR4895611_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895611_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2657477
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG189530.7131952600154207No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT141900.5339651105164785No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC141710.5332501466616645No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC100990.3800221036720167No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78880.29682288877758867No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT58270.21926812536853563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG56830.21384945194257562No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC54320.20440440312371472No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG51970.19556142912996047No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA49080.18468645260147123No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA47230.17772496243617536No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC45180.17001087873949614No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT42360.15939930994699109No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCTGTGGCTCACG41920.15774360417794772No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC39680.14931455662645435No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC39450.1484490740653635No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC35480.13351009246740422No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG34240.12884401257282754No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT33300.12530682297532586No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT30830.11601229286274163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT696750.0136.064471
GGTATCA702750.0134.883658
GTGGTAT703700.0134.5896
GTATCAA706200.0134.285869
TGGTATC709850.0133.493947
AGCAGTG713250.0132.890382
CAGTGGT717550.0132.063924
AGTGGTA722900.0131.074115
GCAGTGG725050.0130.648183
TAAGCAG14650.029.1494981
CAACGCA698850.027.86077910-14
TCAACGC699550.027.81025710-14
TACATGG695750.027.78573420-24
ATCAACG701450.027.6918210-14
CGCAGAG701950.027.63169515-19
AGAGTAC702350.027.53189815-19
CAGAGTA706550.027.48440415-19
GTACATG705750.027.42671620-24
GCAGAGT708850.027.3708515-19
AGTACAT709150.027.27288220-24
Produced by FastQC (version 0.11.5)