FastQCFastQC Report
Sat 18 Mar 2023
SRR4895610_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895610_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2775826
Sequences flagged as poor quality0
Sequence length150
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTCA2778250.0143.872131
GAGTCAA2778400.0143.864362
AGTCAAT1026100.0143.62023
AGTCAAG930200.0143.61213
GTCAATG357650.0142.891894
GTCAATA318350.0142.529054
GTCAAGA272850.0142.257524
GTCAAGT337150.0142.055774
GTCAAGG327900.0142.000964
GTCAATT357250.0141.78224
AGTCAAA834650.0141.488863
GTCAAAT308150.0140.844834
GTCAAAG291350.0140.712374
TCAAGTA112250.0138.931885
TCAATGA99150.0138.3355
TCAATAG114650.0137.405185
TCAAGGT128150.0137.257065
TCAAGAT107000.0136.933755
TCAATGT138300.0135.773575
TCAATTG130100.0135.310475
Produced by FastQC (version 0.11.5)