FastQCFastQC Report
Sat 18 Mar 2023
SRR4895610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2775826
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95420.34375353498382105No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC84830.3056027287012947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT68720.24756594973892454No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCACGGTGGCTCACG63090.22728369861799697No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT51050.18390922197572904No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA44950.16193378115198864No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG42620.15353988326357632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG38860.13999436564107404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC30740.11074181162652125No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAGCTAAGGACTGCAAAA28190.10155535685594126No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA27900.10051062278399293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA545050.0134.029318
GTGGTAT545250.0133.940546
TGGTATC549450.0132.9567
AGTGGTA551200.0132.481645
AAGCAGT552100.0132.356931
GTATCAA555050.0131.692419
AGCAGTG559950.0130.452
CAGTGGT561800.0130.058884
GCAGTGG579650.0125.9916843
CAACGCA539900.027.79230310-14
CGATAGG19700.027.70138435-39
TCAACGC543850.027.55073210-14
ATCAACG544100.027.48779510-14
CGCAGAG546350.027.3488315-19
GTACATG546650.027.27148420-24
TACATGG547250.027.25474420-24
AACGCAG549100.027.21389410-14
AGAGTAC550200.027.0815615-19
AGTACAT551900.026.99640820-24
CAGAGTA555600.026.94274915-19
Produced by FastQC (version 0.11.5)