FastQCFastQC Report
Sat 18 Mar 2023
SRR4895609_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895609_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2391830
Sequences flagged as poor quality0
Sequence length150
%GC21

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC2392600.0143.950971
CGTATCA2393950.0143.86982
GTATCAG820950.0143.674623
GTATCAT846650.0143.480383
GTATCAA729250.0143.436363
TATCATG298050.0142.71884
TATCAGT291200.0142.540344
TATCAGA250800.0142.535024
TATCAGG283550.0142.450214
TATCAAG258150.0142.186234
ATCAGTG99500.0141.538835
ATCAGTA89650.0141.348835
ATCATGA89900.0141.276115
ATCAGGT104850.0141.252355
ATCAGAT90500.0140.816825
ATCAGAG80950.0140.708225
TATCATA264050.0140.536164
ATCAGGG96750.0140.054965
ATCATGT108500.0139.752155
ATCAGGA85700.0139.714435
Produced by FastQC (version 0.11.5)