FastQCFastQC Report
Sat 18 Mar 2023
SRR4895609_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895609_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2391830
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA105890.44271541037615547No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC77370.32347616678442864No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGACTATGAGAATCGAACCC56100.23454844198793393No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT54600.228277093271679No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA45280.18931111324801514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG41450.17329826952584423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG32480.1357956042026398No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC28940.12099522123227822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA28890.12078617627506973No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT28720.12007542342056084No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG27910.11668889511378318No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC27460.1148074904989067No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC27320.11422216461872291No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAA24000.10034157946007868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA483250.0132.756858
GTGGTAT483650.0132.587526
TGGTATC486400.0131.88237
AGTGGTA488450.0131.314065
GTATCAA488700.0131.30589
AAGCAGT489150.0131.153561
CAGTGGT497150.0129.050454
AGCAGTG497800.0128.881932
GCAGTGG503400.0127.419613
CAACGCA477100.027.61191710-14
TCAACGC479300.027.47316410-14
TCGTACG1050.027.42758650-54
GTACATG477900.027.3740120-24
TACATGG478650.027.31005920-24
ATCAACG482450.027.2460310-14
CGCAGAG484100.027.11792615-19
AGAGTAC482600.027.07093415-19
CAGAGTA485900.027.01154315-19
AACGCAG486450.026.95983110-14
AGTACAT485550.026.94865420-24
Produced by FastQC (version 0.11.5)