FastQCFastQC Report
Sat 18 Mar 2023
SRR4895608_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895608_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516838
Sequences flagged as poor quality0
Sequence length150
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACATCG3520100.0143.864212
ACATCGA977500.0143.78563
ACATCGG1054650.0143.780763
ACATCGT1481550.0143.639593
CATCGGT428200.0143.631334
CATCGAT409700.0143.579484
CATCGTA346150.0143.398034
CATCGGA258650.0143.333164
CATCGAA257250.0143.301544
CATCGTG424650.0143.289124
CATCGTT711600.0143.222154
AAACATC3535950.0143.219331
CATCGAG310950.0143.05194
ATCGAGT119800.0142.799245
CATCGGG367350.0142.688054
ATCGATA109800.0142.558615
ATCGATG120450.0142.267755
ATCGTGA107450.0142.259035
ATCGGAT103850.0142.059975
ATCGAAG84500.0141.871055
Produced by FastQC (version 0.11.5)