FastQCFastQC Report
Sat 18 Mar 2023
SRR4895608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516838
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA123430.3509686826632333No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA70230.19969643185156666No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC68360.19437915536626937No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC56170.15971733699419763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG47970.13640093743300089No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG45610.12969036390075403No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG45530.1294628868318643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC44200.12568108056157265No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC42970.12218362062739313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT42880.1219277089248922No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC39670.112800191535692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT828250.0135.691656
GGTATCA829700.0135.497918
AAGCAGT834550.0134.795531
AGTGGTA834500.0134.675385
TGGTATC834800.0134.652887
GTATCAA835850.0134.578469
CAGTGGT846150.0132.874084
AGCAGTG850850.0132.097812
GCAGTGG869550.0129.215593
CAACGCA822750.028.0583110-14
TCAACGC825700.027.93539410-14
GTACATG827500.027.74973720-24
ATCAACG830600.027.7203210-14
CGCAGAG830150.027.70948415-19
TACATGG828450.027.676220-24
AACGCAG831300.027.63635410-14
AGAGTAC831350.027.5707615-19
CAGAGTA837850.027.49779715-19
AGTACAT835250.027.46294820-24
GCAGAGT839700.027.41320615-19
Produced by FastQC (version 0.11.5)