FastQCFastQC Report
Sat 18 Mar 2023
SRR4895607_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895607_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165452
Sequences flagged as poor quality0
Sequence length150
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGA1165800.0143.956472
ACGCTCG1165950.0143.937941
GCTCGAG397350.0143.890983
GCTCGAA353450.0143.877473
GCTCGAT413100.0143.842833
CTCGAGT136600.0143.736164
CTCGATA128500.0143.663514
CTCGATG141650.0143.593064
CTCGAGA121350.0143.584374
CTCGAAG125900.0143.485024
CTCGATT142850.0143.445284
TCGAGAT43200.0143.166375
CTCGAGG138150.0143.165834
CTCGAAT128650.0143.160224
TCGATAG47150.0143.083485
TCGATGG48850.0142.820575
TCGAGTA44000.0142.690615
TCGAGTG43800.0142.684635
TCGATAT43700.0142.681645
CTCGAAA100150.0142.633744
Produced by FastQC (version 0.11.5)