FastQCFastQC Report
Sat 18 Mar 2023
SRR4895607_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895607_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165452
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG39320.3373798320308344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCCAGGCGCGGTGGCTC36900.31661535610218183No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA34390.2950786475976702No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC34380.2949928439781304No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG33000.283151944481626No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30100.2582688948150589No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCATGGTGGCTTAC22160.19014082090038886No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA18700.1604527685395881No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT17190.1474964219890652No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTGGGCGTGGTGGCTCACG15900.1364277550684198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCGGTGGCTCACGCCT14370.12329980127881715No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAGCTAAGGACTGCAAAA13880.11909542392136269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA245500.0130.913858
GTGGTAT246000.0130.53076
AAGCAGT247100.0130.3521
TGGTATC247850.0129.61457
GTATCAA249450.0128.898599
AGCAGTG252050.0127.5403752
AGTGGTA252050.0127.454685
CAGTGGT252650.0127.266014
GCAGTGG257650.0124.7682953
CAACGCA243200.027.01062810-14
ATCAACG243400.026.89969310-14
GTACATG243200.026.85668820-24
TCAACGC245100.026.78949410-14
AGTACAT244650.026.67985520-24
TACATGG245600.026.57079320-24
AGAGTAC245450.026.56943115-19
CGCAGAG245850.026.567215-19
CAGAGTA246900.026.50670815-19
GAGTACA243100.026.43533920-24
GCAGAGT247250.026.43423815-19
Produced by FastQC (version 0.11.5)