FastQCFastQC Report
Sat 18 Mar 2023
SRR4895606_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895606_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561539
Sequences flagged as poor quality0
Sequence length150
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCCAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT38710.24789646624259784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCCA1569250.0143.288161
CCTCCAA1572800.0142.964742
CTCCAAG548700.0142.857733
CTCCAAT562500.0142.693733
TCCAATG197550.0141.593864
TCCAAGA162850.0140.550774
CTCCAAA470300.0140.080153
TCCAATA171150.0140.002854
CCAATAG59050.0139.975655
TCCAAGT202600.0139.876944
CCAATGT75000.0138.815355
TCCAAGG194250.0138.55074
CCAAGAT60400.0138.15835
CCAAGTA66500.0138.152745
CCAATGA58950.0138.014625
CCAATGG68150.0137.66045
TCCAAAG170450.0137.15634
CCAATAT63600.0134.489935
TCCAATT209450.0134.30544
CCAAGAG58250.0133.987355
Produced by FastQC (version 0.11.5)