FastQCFastQC Report
Sat 18 Mar 2023
SRR4895606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561539
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG83570.5351771553576311No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT57850.37046785254803116No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC49510.3170590039698016No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43730.28004423840839066No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC43370.27773882048415055No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG40820.26140877685411634No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCACGGTGGCTCA39210.25109843558182027No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT26330.16861570540345133No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG25220.16150733347037763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCAGTGGCTCAC24130.15452704031087278No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA23820.1525418193205549No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCCAGCGCGGTGGCTCA23760.15215758299984825No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA19360.12398025281469116No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCGTGGTGGCTCATG19340.12385217404112225No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAGCTAAGGACTGCAAAA19050.12199503182437327No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC18760.12013788960762427No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC18760.12013788960762427No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCACGGTGGCTCAC17960.11501473866486844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG17420.11155661177850826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT383600.0127.513956
GGTATCA385250.0126.986518
TGGTATC387000.0126.4308857
AAGCAGT387100.0126.363921
GTATCAA388400.0125.99379
AGCAGTG389850.0125.4736942
AGTGGTA392300.0124.796215
CAGTGGT392150.0124.7928544
GCAGTGG405000.0120.7622763
CGATAGG17100.026.9460235-39
CATGGGG226600.026.56813225-29
TCGTACG1250.026.49467550-54
CAACGCA380400.026.28133410-14
GTACATG381550.026.05220-24
TACATGG381600.026.04481520-24
TCAACGC384050.025.98281510-14
CGCAGAG384050.025.91565915-19
ATCAACG384550.025.90784310-14
CAGAGTA388550.025.67110815-19
AGAGTAC387300.025.66844715-19
Produced by FastQC (version 0.11.5)