FastQCFastQC Report
Sat 18 Mar 2023
SRR4895605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327502
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50050.377023914088265No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT40520.30523494503209786No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA37330.2812048494088898No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG26140.1969111910942507No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA25200.18983022247800754No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG22700.17099785913693538No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC22090.16640276248171376No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG20840.1569865808111777No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC19760.1488509998478345No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC19040.14342727920560572No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATTCTTCGCCAGGCTCTCT17120.1289640241596623No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGTGGTGGCTCACGCCT14740.11103561425896158No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC14230.10719381213738284No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTTCGCCAGGCTCTCTG14220.10711848268401855No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC14130.10644051760373996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT307050.0134.140186
AAGCAGT308850.0133.447341
GGTATCA309700.0133.038888
GTATCAA311450.0132.314459
TGGTATC312750.0131.741467
AGTGGTA312900.0131.701315
AGCAGTG315800.0130.4742
CAGTGGT316250.0130.379434
GCAGTGG323550.0127.304253
TCGTACG1550.027.86967750-54
CAACGCA306100.027.58866710-14
ATCAACG307250.027.452610-14
TCAACGC307950.027.41357610-14
TACATGG306000.027.36814520-24
GTACATG306800.027.36248820-24
CGCAGAG308650.027.33315515-19
AGTACAT309000.027.16767320-24
AGAGTAC309500.027.081315-19
CAGAGTA312400.026.95896115-19
AACGCAG313200.026.87130710-14
Produced by FastQC (version 0.11.5)