FastQCFastQC Report
Sat 18 Mar 2023
SRR4895604_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895604_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4046434
Sequences flagged as poor quality0
Sequence length150
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAGAA4058200.0143.579182
ACACAGA4059050.0143.54911
ACAGAAT1461100.0143.09613
ACAGAAG1382600.0143.03973
CAGAAGT502400.0142.378464
CAGAATG499050.0142.05024
CAGAATA458650.0141.40774
CAGAAGA423800.0140.60014
CAGAAGG470800.0140.251114
ACAGAAA1251200.0139.820183
CAGAATT521600.0139.787834
CAGAAAG447700.0139.575334
CAGAAAT463400.0139.259064
AGAATAG172700.0130.990545
AGAATGA165800.0128.538515
AGAAGTA174800.0128.387135
AGAATGG188150.0128.19375
AGAATTG198850.0126.364745
AGAAGTG178550.0126.335855
CAGAAAA379000.0125.60874
Produced by FastQC (version 0.11.5)