FastQCFastQC Report
Sat 18 Mar 2023
SRR4895604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4046434
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC137530.33987950872298917No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA129260.31944176032526417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT79040.1953324828725737No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC78950.19511006481262266No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG77090.1905134249069675No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC75130.18566965382358888No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG64210.1586829292161938No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG57390.14182858289545808No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT53120.13127608160666898No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA45450.11232112027528436No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTCTGGGCTCGGACCTA42760.10567329159452496No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC42040.10389394711491648No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGTGGGGTTTTCTTCTA41750.10317726669951863No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC40890.10105193857109741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT977600.0135.956276
GGTATCA983450.0135.271998
AAGCAGT988500.0134.676221
GTATCAA991400.0134.267149
TGGTATC992850.0133.976787
AGTGGTA995500.0133.605685
AGCAGTG1001950.0132.696932
CAGTGGT1005750.0132.267154
GCAGTGG1042250.0127.552213
CAACGCA972900.028.0389410-14
TCAACGC976750.027.90336210-14
TACATGG979950.027.66079520-24
GTACATG981750.027.65848420-24
AACGCAG983600.027.60802710-14
CGCAGAG985800.027.593715-19
ATCAACG989200.027.52305810-14
AGTACAT991500.027.38214720-24
AGAGTAC990800.027.34981315-19
GCAGAGT994850.027.33689515-19
CAGAGTA998800.027.27347815-19
Produced by FastQC (version 0.11.5)