FastQCFastQC Report
Sat 18 Mar 2023
SRR4895603_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895603_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2789853
Sequences flagged as poor quality0
Sequence length150
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTAC2794250.0143.772691
AGGTACA2795150.0143.72642
GGTACAT1010400.0143.251223
GGTACAG939100.0143.056413
GGTACAA854600.0142.727263
GTACAGT347500.0142.569824
GTACATG340350.0142.370544
GTACAAG286350.0142.289644
GTACATA315300.0142.195454
GTACAGA279250.0141.833664
GTACATT361250.0141.56794
GTACAAT309650.0141.395224
GTACAGG316150.0141.221044
TACATAG109100.0139.511835
TACAGTA107850.0138.925755
TACATGA107450.0138.236795
TACAGTG118550.0138.229755
TACAGAT102600.0137.824025
TACAGAG94250.0137.735285
TACATGG122150.0137.574585
Produced by FastQC (version 0.11.5)