FastQCFastQC Report
Sat 18 Mar 2023
SRR4895603_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895603_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2789853
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85230.30549996720257305No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG48560.17405935008045229No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG47670.17086921784050987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT40200.14409361353447656No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC35530.1273543803203968No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC32420.11620683957183407No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTCTGGGCTCGGACCTAG29970.10742501486637468No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCTCGGCCTTAGCGCCATT29420.10545358483045522No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTCTGGGCTCGGACCTA28920.10366137570689209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT771600.0136.199076
AAGCAGT773900.0135.83851
GGTATCA775050.0135.68578
TGGTATC777650.0135.278347
AGTGGTA780200.0134.725465
GTATCAA781700.0134.577459
CAGTGGT787000.0133.570534
AGCAGTG790950.0132.885272
GCAGTGG805800.0130.382743
CAACGCA769700.028.0104510-14
TCAACGC772100.027.90473610-14
ATCAACG775200.027.75599510-14
GTACATG773300.027.7520720-24
AACGCAG777050.027.64358910-14
CGCAGAG777900.027.6339415-19
TACATGG775400.027.63048420-24
AGTACAT777850.027.55456220-24
AGAGTAC780900.027.48905815-19
CAGAGTA783650.027.47711415-19
GCAGAGT784800.027.41315-19
Produced by FastQC (version 0.11.5)