FastQCFastQC Report
Sat 18 Mar 2023
SRR4895602_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895602_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069088
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG64120.3098949875500704No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60270.2912877557648587No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA45060.21777710759523036No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT44690.21598888012496326No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC41980.20289132216706104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT38340.18529903029740638No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA36260.17524629208617518No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG35520.17166983714564096No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG30390.14687630492274856No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC30250.14619967831237723No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGTGGGGTTTTCTTCTA27820.13445537357521767No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC27030.13063726627383657No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC26740.12923568258092455No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCACGGTGGCTCA25070.12116449372863794No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCGGGCGCGGTGGCTCA24440.11811967398196693No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCCAGGCACGGTGGCTC24300.1174430473715956No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC21760.10516710744057284No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC21410.10347554091464453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT492850.0135.089311
GGTATCA493600.0134.786218
GTGGTAT493900.0134.616886
TGGTATC497200.0133.752387
AGTGGTA500050.0133.105245
GTATCAA501550.0132.735879
AGCAGTG503300.0132.206012
CAGTGGT505950.0131.58474
GCAGTGG522500.0127.320353
CAACGCA489550.027.95381210-14
TCAACGC492450.027.76287810-14
ATCAACG493250.027.65654810-14
GTACATG493900.027.5858320-24
CGCAGAG495750.027.52605815-19
TACATGG493900.027.51294520-24
TACGCAC8250.027.40248550-54
AACGCAG498550.027.35098310-14
AGTACAT498400.027.28764720-24
GCAGAGT501400.027.244615-19
GAGTACA493900.027.03481520-24
Produced by FastQC (version 0.11.5)