FastQCFastQC Report
Sat 18 Mar 2023
SRR4895601_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895601_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910046
Sequences flagged as poor quality0
Sequence length150
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA1911250.0143.905642
CGCTTAG636800.0143.717153
CGCTTAT679150.0143.554553
CGCTTAA597200.0143.553733
AACGCTT1916450.0143.515171
GCTTATG235800.0142.747914
GCTTATA209550.0142.591084
GCTTAGT228050.0142.515934
GCTTAGA196250.0142.458924
GCTTAAG207750.0142.40564
GCTTAGG216850.0142.273284
GCTTAAT210200.0139.957964
CTTAGTA76850.0139.783815
GCTTATT242450.0139.307724
GCTTAAA190700.0139.242614
CTTAGGA70150.0139.073245
CTTAGTG73350.0138.797385
CTTAGAG64650.0138.654135
CTTATGA71350.0138.550635
CTTATAG80700.0138.468235
Produced by FastQC (version 0.11.5)