FastQCFastQC Report
Sat 18 Mar 2023
SRR4895601_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895601_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910046
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54370.2846528303506827No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA45540.23842357723321844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC45310.23721941775224262No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT32660.1709906462985708No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT29440.15413241356490892No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACGCGCTTCAACTTCGGT26130.13680298799086513No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG26080.13654121419065302No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC24730.1294733215849252No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG23410.12256249325932464No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA22950.12015417429737293No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG22370.11711759821491209No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTTCGCCAGGCTCTCTG22200.11622756729419083No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA20450.10706548428676588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT486200.0135.758971
GTGGTAT486800.0135.560886
GGTATCA487800.0135.474858
AGTGGTA490150.0134.678445
TGGTATC490900.0134.560687
GTATCAA492950.0134.132529
CAGTGGT495350.0133.264624
AGCAGTG497250.0132.668552
GCAGTGG506450.0130.230123
TAAGCAG13050.029.9614221
CAACGCA482700.028.07927510-14
TCAACGC486850.027.82513210-14
ATCAACG487050.027.76640510-14
TACATGG485900.027.7498720-24
CGCAGAG487050.027.73713115-19
GTACATG487600.027.67674620-24
AACGCAG488500.027.61029610-14
AGTACAT491150.027.44151920-24
AGAGTAC491100.027.43801915-19
CAGAGTA494300.027.4031315-19
Produced by FastQC (version 0.11.5)