FastQCFastQC Report
Sat 18 Mar 2023
SRR4895600_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895600_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987094
Sequences flagged as poor quality0
Sequence length150
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAG1988750.0143.876391
ACCGAGA1988750.0143.876392
CGAGATA216450.0143.766624
CCGAGAT690950.0143.697283
CCGAGAG683500.0143.630783
CGAGAGT233100.0143.628814
CGAGATG241100.0143.402224
CGAGATT232850.0143.31924
CCGAGAA615550.0143.285963
CGAGAAT216200.0143.266824
CGAGAGA211500.0143.04634
CGAGAAG213550.0143.021714
CGAGAGG238400.0142.4294
CGAGAAA188150.0141.05294
GAGAGTA89250.0124.718875
GAGATTA86400.0123.499555
GAGAATG89400.0122.8183445
GAGAGTG89950.0122.227455
GAGATGA84300.0122.134775
GAGATAG86150.0122.019285
Produced by FastQC (version 0.11.5)